Direct and multiplex quantification of protein biomarkers in serum samples using an immuno-magnetic platform.

Direct and multiplex quantification of protein biomarkers in serum samples using an immuno-magnetic platform† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc04115e Click here for additional data file.

Integrated analyses of proteins and their glycans in a magnetic bead-based multiplex assay format.

BACKGROUND Well-annotated clinical samples are valuable resources for biomarker discovery and validation. Multiplex and integrated methods that simultaneously measure multiple analytes and generate integrated information about these analytes from a single measurement are desirable because these methods help conserve precious samples. We developed a magnetic bead-based system for multiplex and i...

Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients

The role of microRNAs (miRs) is shown as a biomarker whose expression level changes have been associated with cancer development and progression. Circulating miRNAs have been postulated as biomarkers for breast cancer. Detection and diagnosis of miRNA markers could provide an improved and sensitive method in the clinical application. In this study, we applied and improved the effective approach...

The Role of Inflammatory Biomarkers in the Management of Children with Asthma

Background Making decision on asthma as an inflammatory disease is done mostly on the airways function and the patient symptoms which most of them are nonspecific and subjective. Also, the children are not able to express their signs and collaboration in examination of the lungs function. Evaluation of serum level inflammatory biomarkers can be useful in assessment of the response to treatment ...

شناسایی پروتئینهای لارو فیلاریفرم (L3) استرونژیلوئیدس استرکورالیس به روش وسترن بلات

Background: Strongyloides stercoralis is prevalent in tropical and subtropical regions of the world. S. stercoralis is the only nematodes with the ability to multiply in its host's body via autoinfection transmission. Larvae detection in faeces is difficult partly because of low egg production and irregular larvae excretion in faeces. Serologic tests (ELISA, IFA) are also diagnostic, however, S...